Bottacelli's "Loss of Innocence"

Innocence is the idea of being pure and naive and not yet jaded or tainted

You can't loose innocence in a sense that it's an object you can't find anymore but you can do or say something that makes you experienced or knowing.

One artistic technique that the artist used was the choice to show two differing sides next to the girl who was the main focus of the painting. I interpreted these sides as innocence and loss of innocence.
Another technique I the Bottacelli used was having the main girl turned slightly to the "loss of innocence" side, perhaps foreshadowing her choice?
The third technique was the choice of position of surroundings the main girl. For example Bottacelli placed her standing in a giant clam in the ocean. This places emphasis and importance on the girl herself. It allows the audience to see that she is the main focus of the painting.